
- MultiSpecies
- USES-WesternblottingandChIP
- APPLICATIONS-ReactswithmammalianLysineSpecificDemethylase1
AbX™LSDRabbitPolyclonalAntibodyforWesternblottingandChIP.
Therearetwoknownclassesofhistonedemethylases(HDM),differingintheirreactionchemistry,coenzymeuseandreactionproducts.Flavin-dependentHDMsactonlyonmono-anddimethylatedlysinesandproducehydrogenperoxide,whereastheJumonji-containingHDMsareiron-dependentenzymesthatcanactonmono-,di-andtrimethylatedlysinesidechains,andalsoonmethylatedArginineresidues.Bothclassesalsoproduceformaldehydeasaproductofthedemethylationreaction.LSD1(Lysine-specificdemethylase1)isaflavindependentamineoxidaseandwasidentifiedasasubunitofdifferentcomplexesandhasbeenshowntobeinvolvedintranscriptionalrepressionofgenesthroughthedemethylationofmonomethylLys4anddimethylLys4HistoneH3.LSD1isalsoinvolvedintranscriptionalactivationthroughthedemethylationofmonomethylLys9anddimethylLys9HistoneH3.Itisalsoabletodemethylatebothmono-anddimethylLys370intheregulatorydomainofthetumorsuppressorp53.