
- RabbitAntibodytowildtypeGreenFluorescentProtein(GFP)
- ProteinAPurified
- ReactswithallGFPvariants
Greenfluorescentprotein(GFP),a27kDaproteinderivedfromthejellyfishAequoreavictoria,emitsgreenlight(emissionpeak509nm)whenexcitedbybluelight(excitationpeak395nm). GFPhasbecomeaninvaluabletoolincellBIOLOGyresearch,sinceitsintrinsicfluorescencecanbevisualizedinlivingcells.GFPfluorescenceisstableunderfixationconditionsandsuitableforavarietyofapplications.GFPhasbeenwidelyusedasareporterforgeneexpression,enablingresearcherstovisualizeandlocalizeGFP-taggedproteinswithinlivingcellswithouttheneedforchemicalstaining.OtherapplicationsofGFPincludeassessmentofprotein-proteininteractionsthroughtheyeasttwohybridsystemandmeasurementofdistancebetweenproteinsthroughfluorescenceenergytransfer(FRET)protocols.GFPisusedtomeasuresinglecellmetastasisandsuccessfulproliferationofstemcells.