
- MultiSpecies
- VERSATILE-Westernblotting,ImmunoassayandImmunoprecipitation
- PURE-SuppliedlabeledinPBSwithnocarrierprotein
- BRIGHT-Strongfluorescence-3moleDyLight488/IgG
TheARBORASSAYSAbX™DyLight®488-labeledGlutathione(GSH)MouseMonoclonalAntibodyisproducedfromourProteinApurifiedantibodybytreatmentwithDyLight®488NHSester.ItwillfluorescentlylabelGSH-proteincomplexesundernon-reducingconditions.SimilarfluorescentpropertiestoFITC.
Glutathione(GSH)isthehighestconcentrationnon-proteinthiolinmammaliancellsandispresentinconcentrationsof0.5to10mM.GSHplaysakeyroleinmanyBIOLOGicalprocesses,includingthesynthesisofproteinsandDNA,thetransportofaminoacids,andtheprotectionofcellsagainstoxidation.Harmfulhydrogenperoxidecellularlevelsareminimizedbytheenzymeglutathioneperoxidase(GP)usingGSHasareductant.TheoxidizedGSHdimer,GSSG,isformedfromGSHandperoxidebytheGPreaction.Animportantroleof GSSGintheNFkBactivatingsignalcascadeissuggestedbythefactthatthepotentNFkBinducerTPAincreasesintracellularGSSGlevelsandGSSG/GSHratios.