
- MultiSpecies
- MULTI-FORMAT-StandardRangeof50-0.8ng/mLor4,000-63pg/mL
- SAMPLE-Serum,Plasma,Urine,MediaandDriedFecalExtracts
- SAMPLES/KIT-39or231inDuplicate
- SENSITIVITY-Measure<0.3=""ng/ml=""in=""10ul="">
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TheDetectX®Thyroxine(T4)EnzymeImmunoassaykitisdesignedtoquantitativelymeasureT4presentinserum,EDTAplasma,tissueculturemediasamplesandfecalextracts. Athyroxinestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturemouseantibodies.Athyroxine-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofamonoclonalantibodytoT4toeachwell.Afteranhourincubationtheplateiswashedandsuppliedsubstrateisadded.ThesubstratereactswiththeboundT4-peroxidaseconjugate.Aftera30minuteincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheT4inthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
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