- MostSensitive
 - MultiSpecies
 
- USE-MeasureThiolContentofRecombinantProteins
 - SAMPLE-Buffers
 - SAMPLES/KIT-39inDuplicate
 - SENSITIVE-Measure<0.5=""pmol="">
 - STABILITY-Stable4˚CLiquidReagents
 
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TheDetectX®ThiolFluorescentDetectionkitallowsuserstoaccuratelydeterminetheextentoffreethiolcontentinsamplesusingaproprietarysubstrate,ThioStar®,thatisconvertedtoabrightlyfluorescentproductuponreactionwiththiolsinthesample.Thefluorescentsignalisreadat510nmwithexcitationat410nminafluorescentplatereader.Thethiolinthesamplecaneitherbeonethatisgeneratedbyareaction,suchastheendproductofanenzymaticreactionthatproducesreducedglutathione,orcanbethecysteinecontentoftheproteintobemeasured.Thegraphshowsthemeasurementofthiolcontentinasamplecontainingupto4MguanidineHCl,equivalentto8MGuHClinthesample.
FreethiolsinBIOLOGicalsystemshaveimportantroles.Oxidativelymodifiedthiolgroupsofcysteineresiduesareknowntomodulatetheactivityofagrowingnumberofproteins.Oneofthemostpressingproblemswiththisapproachistoaccuratelydeterminetheextentofmodificationofspecificaminoacids,suchascysteineresidues,inacomplexproteinsample.

DataReductionTemplatebyMyAssays

		