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- MostSensitive
- MultiSpecies
- USE-MeasureThiolContentofRecombinantProteins
- SAMPLE-Buffers
- SAMPLES/KIT-39inDuplicate
- SENSITIVE-Measure<0.5=""pmol="">
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®ThiolFluorescentDetectionkitallowsuserstoaccuratelydeterminetheextentoffreethiolcontentinsamplesusingaproprietarysubstrate,ThioStar®,thatisconvertedtoabrightlyfluorescentproductuponreactionwiththiolsinthesample.Thefluorescentsignalisreadat510nmwithexcitationat410nminafluorescentplatereader.Thethiolinthesamplecaneitherbeonethatisgeneratedbyareaction,suchastheendproductofanenzymaticreactionthatproducesreducedglutathione,orcanbethecysteinecontentoftheproteintobemeasured.Thegraphshowsthemeasurementofthiolcontentinasamplecontainingupto4MguanidineHCl,equivalentto8MGuHClinthesample.
FreethiolsinBIOLOGicalsystemshaveimportantroles.Oxidativelymodifiedthiolgroupsofcysteineresiduesareknowntomodulatetheactivityofagrowingnumberofproteins.Oneofthemostpressingproblemswiththisapproachistoaccuratelydeterminetheextentofmodificationofspecificaminoacids,suchascysteineresidues,inacomplexproteinsample.
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