
- MultiSpecies
- USE-MeasureSODActivity
- SAMPLE-Serum,Plasma,Cells,TissuesandErythrocyteLysates
- SAMPLES/KIT-89inDuplicate
- STABILITY-Stable4˚CLiquidReagents
ViewPublications
TheDetectX®SuperoxideDismutase(SOD)ActivityKitisdesignedtoquantitativelymeasureSODactivityinavarietyofsamples.TheassaymeasuresalltypesofSODactivity,includingCu/Zn,Mn,andFeSODtypes.AbovineerythrocyteSODstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffofthestandardcurve.SamplesaredilutedinourspeciallycoloredSampleDiluentandaddedtothewells.TheSubstrateisaddedfollowedbyXanthineOxidaseReagentandincubatedatroomtemperaturefor20minutes.TheXanthineOxidasegeneratessuperoxideinthepresenceofoxygen,whichconvertsacolorlesssubstrateintheDetectionReagentintoayellowcoloredproduct.Thecoloredproductisreadat450nm.IncreasinglevelsofSODinthesamplescausesadecreaseinsuperoxideconcentrationandareductioninyellowproduct.TheresultsareexpressedintermsofunitsofSODactivitypermL.
DataReductionTemplatebyMyAssays