
- MultiSpecies
- USE-VitaminADeficiencyMeasuredin90Minutes
- SAMPLE-Serum,PlasmaandDriedBloodSpots(DBS)
- SAMPLES/KIT-38inDuplicate
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®RetinolBindingProtein(RBP)ImmunoassayKitisdesignedtomeasureRBPlevelsinserum,EDTAandheparinplasma.ThiskitusesnativehumanRBPasastandard,theconcentrationofwhichhasbeenestablishedviaaminoacidanalysis.StandardsordilutedsamplesarePipettedintoamicrotiterplatecoatedandRBP-peroxidaseconjugateisadded.ThebindingreactionisinitiatedbyadditionofaRBP-specificrabbitpolyclonalantibody.Aftera60-minuteincubation,theplateiswashedandTMBsubstratesolutionaddedtoeachwell.After30minutestheintensityofthegeneratedsignalisreadat450nminamicrotiterplatereader.
Retinolbindingprotein(RBP)isfromafamilyofstructurallyrelatedproteinsthatbindsmallhydrophobicmoleculessuchasbilepigments,steroids,odorants,etc.RBPisahighlyconserved21kDasingle-chainglycoprotein,consistingof182aminoacidswith3disulfidebonds,thathasahydrophobicpocketwhichbindsretinol(vitaminA).RBPbindsretinolina1:1stoichiometry,whichservestonotonlysolubilizeretinolbutalsoprotectitfromoxidation.Wheninserum,themajorityofRBPboundwithretinolisreversIBLycomplexedwithtransthyretin.Thiscomplextransportsretinoltospecificreceptorsofvarioustissuesinthebody.VitaminAstatusisreflectedbyserumconcentrationasitishomeostaticallycontrolledanddoesnotfalluntilstoresaredramaticallyreduced.RBPhasbeenshowntobeausefulsurrogateMarkerforretinolbecauseofthecorrelationbetweenretinolandRBPinserum,whichimpliesthatRBPmaybeusedtomonitorvitaminAdeficiency(VAD).TheWHOhasestimatedthat250millionchildrenhavemoderatetosevereVADduetolackofadequatenutrition,andtherisingcostoffoodstaplesaroundtheworldfurtherexacerbatesthisproblem.Inadditiontonutritionaldeficiencies,infectiousstresseshavebeenshowntodepressretinolconcentrationsandindividualswithdiseasessuchascysticfibrosisandHIV-1alsoruntheriskofVADduetotheinfectiousstressesthatcontributetothedisease.ModulatingRBP4levelsmayleadtonewstrategiesintreatingtype2diabetes.
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