
- MostSensitive
- MultiSpecies
- QUICK-Resultsin90Minutes
- Convenient-OnekitforthemeasurementofabroadrangeofRBPconcentrations
- SAMPLE-Serum,Plasma,Urine,DriedBloodSpots
- SAMPLES/KIT-38or230inDuplicate
- Stable4˚CLiquidReagents
TheDetectX® RetinolBindingProtein(RBP)Multi-FormatEIAKitisdesignedtoallowforthemeasurementofbothhighandlowRBPlevelsinavarietyofsamplesincludingdriedbloodspots,serum,EDTAandheparinplasmaandurinewiththesamekit.ThekitusesnativehumanRBPasastandard.StandardsordilutedsamplesareaddedtoacoatedmicrotiterplatealongwithaRBP-peroxidaseconjugateandthebindingreactioninitiatedbyadditionofaRBP-specificsheepantibody.Aftera60-minuteincubation,theplateiswashedandTMBsubstratesolutionadded.After30minutes,thecolordevelopmentisstoppedandtheintensityofthegeneratedsignalisreadat450nm.
Retinolbindingprotein(RBP)isfromafamilyofstructurallyrelatedproteinsthatbindsmallhydrophobicmoleculessuchasbilepigments,steroids,odorants,etc.RBPisa21kDahighlyconserved,single-chainglycoprotein,consistingof182aminoacidswith3disulfidebonds,thathasahydrophobicpocketwhichbindsretinol.InurineRBPhasbeenshowntobeausefulMarkerforrenalfunctionasitistotallyfilteredbytheglomeruliandreabsorbedbyproximaltubules.ThishasmadeRBPatooltostudyrenalfunctioninheartorkidneytransplantrecipients,type1and2diabetics,andinpeopleexposedtouraniumfromminingoperations. ModulatingRBP4levelsmayleadtonewstrategiesintreatingtype2diabetes
Wheninserum,themajorityofRBPboundwithretinolisreversIBLycomplexedwithtransthyretin. Thiscomplextransportsretinoltospecificreceptorsofvarioustissuesinthebody. VitaminAstatusisreflectedbyserumconcentrationasitishomeostaticallycontrolledanddoesnotfalluntilstoresaredrasticallyreduced. RBPhasbeenshowntobeausefulsurrogatemarkerforretinolbecauseofthecorrelationbetweenretinolandRBPinserum,whichimpliesthatRBPmaybeusedtomonitorvitaminAdeficiency(VAD). TheWHOhasestimatedthat250millionchildrenhavemoderatetosevereVADduetolackofadequatenutrition,andtherisingcostoffoodstaplesaroundtheworldfurtherexacerbatesthisproblem. Inadditiontonutritionaldeficiencies,infectiousstresseshavebeenshowntodepressretinolconcentrationsandindividualswithdiseasessuchascysticfibrosisandHIV-1alsoruntheriskofVADduetotheinfectiousstressesthatcontributetothedisease. MeasurementofRBPlevelshasalsobeenusefulindetectionandcharacterizationofdiseasesincludinghypertensionandcertaincancers,amongotherconditions.
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