>
产品中心 >
Other_kits >
ARBOR ASSAYS/Protein Kinase A (PKA) Colorimetric Activity Kit/K027-H1/H1

- MostSensitive
- MultiSpecies
- USE-MeasurePKAActivityin3Hours
- SAMPLE-Lysates,purifiedsystems
- SAMPLES/KIT-42inDuplicate
- STABILITY-PKAStandardStoredat-20˚C
ViewPublications
TheDetectX®cAMP-DependantProteinKinaseA(PKA)ActivitykitisdesignedtoquantitativelymeasurePKAactivityinavarietyofsamples.RecombinantPKAstandardisprovidedtogenerateastandardcurvefortheassay.ForsamplesthatmayhavehighPKAactivityweofferanalternativeprotocolthatreadsthesubstratereactionat650nm.ThekitutilizesanimmobilizedPKAsubstrateboundtoamicrotiterplate.SamplescontainingPKAwill,inthepresenceofthesuppliedATP,phosphorylatetheimmobilizedPKAsubstrate.Arabbitantibodyspecificforthephospho-PKAsubstratebindstothemodifiedimmobilizedsubstrate.AntibodyspecificforrabbitIgGlabeledwithperoxidaseissimultaneouslyaddedtotheplatetobindtotherabbitanti-phospho-PKAsubstrate.Afterashortincubationandwash,substrateisaddedandtheintensityofthecolordevelopedisdirectlyproportionaltotheamountofPKAinthesamplesandstandards.
DataReductionTemplatebyMyAssays