- MostSensitive
- MultiSpecies
- USE-MeasureProlactininSerumandBIOLOGicalSamples
- SAMPLE-Serum,Plasma,Urine,BufferandTCM
- SAMPLES/KIT-40inDuplicate
- SENSITIVE-Measures<12=""pg/ml="">
TheDetectX® ProlactinImmunoassaykitisdesignedtoquantitativelymeasureProlactinpresentinbiologicalsamplesandtissueculturemedia. Thekithasbeentestedtomeasurehumanandelephantprolactin. Otherspecieshavenotbeenmeasured.AhumanProlactinstandardisprovidedtogenerateastandardcurvefortheassay. StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithamonoclonalantibodytoProlactin.Aftera60minuteincubation,theplateiswashedandaperoxidaseconjugatedProlactinpolyclonalantibodyisadded. Theplateisagainincubatedfor60minutesandwashed. Substrateisthenaddedtotheplate,whichreactswiththeboundProlactinAntibodyConjugate. Afterathirdincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.
DataReductionTemplatebyMyAssays