
- MultiSpecies
- USE-Measurepregnancycoursein90Minutes
- SAMPLE-FecalExtracts,Urine,Serum,PlasmaandTCM
- SAMPLES/KIT-39or231inDuplicate
- SELECTIVE-NoCrossReactivitytoOtherRelatedPGFMetabolites
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®13,14,Dihydro-15-keto-ProstaglandinF2alpha(PGFM)EnzymeImmunoassaykitmeasuresPGFMpresentinnon-invasivelycollectedfecalandurinesamples.TheassaywillalsomeasurePGFMinplasma,serumandTCMsamples.ThekithasbeendevelopedforurineandextractedfecalsamplesandhasbeenvalidatedwithIberianlynxandotherfelidsamples.Afteranhourincubationtheplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundPGFM-peroxidaseconjugateandthereactionisreadat450nmwavelength.
PGFMhasbeensuggestedasausefulnon-invasivepregnancy Markerbothinurineandfecalsamples.Ithasbeenshowntobeaprecise,practicalmethodforthisapplicationinthesematricesforanumberofspecies.ParallelcourseswereobtainedwhencomparingurinaryandfecalPGFMinavarietyoffelidsandotherspecies,andonlyasimpledilutionoffecalextractsisnecessarypriortoanalyses.FecalPGFManalysesmayallowpregnancydiagnosisincaptiveandfree-rangingfelids.RecentevidencehassuggestedthatPGFMmayalsobeausefulpregnancymarkerinsomeothernon-felidspecies.
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