- MostSensitive
- MultiSpecies
- USE-MeasurePGE2between1,000-2pg/mL
- SAMPLE-Serum,Plasma,Saliva,UrineandTCM
- CONVENIENT-Onekitforrapid,sensitive,lowsamplevolumes
- SAMPLES/KIT-upto39or231inDuplicate
- SENSITIVE-Measure<0.2=""pg=""per="">
- VERSATILE-WorksdirectlywithMouseandRatSerum
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TheDetectX®ProstaglandinE2(PGE2)Multi-FormatEnzymeImmunoassaykitisdesignedtoquantitativelymeasurePGE2presentinserum,plasma,urine,tissues,salivaandculturemediasamples. ThisoneEIAkitallowsallsamplestobemeasuredwithjustonekit. Themonoclonalantibodyhassuper-fastkineticsandshowsthesamebindingcharacteristicsat2or16hourincubation. Thekitallowssamplesaslowas2pg/mLtobemeasuredinlessthan3hours.Itexhibitsexcellentcrossreactivityandlowsamplerequirements.
APGE2standardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturemouseantibodies.APGE2-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionofamonoclonalantibodytoPGE2.Aftereitheranincubationtheplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundPGE2-peroxidaseconjugate.Aftera30minuteincubationtheintensityofthegeneratedcolorisreadat450nm.
The new PGE2 monoclonal (3H10)developedto useinthenew PGE2 EIAkitshassometrulyamazingproperties:
- SuperFastKinetics–SameAssayRangeat2or16hours
- IncredIBLeSensitivity–LowestStandard<2pg/mL
- SuperiorCrossReactivitytoOtherEicosanoids
- SuperLowSampleVolumeRequirements
- ChoiceofIncubationTime,Sensitivity,andSampleVolumeinaSingleKit
DataReductionTemplatebyMyAssays
