
- MostSensitive
- N-CALKit
- USE-MeasurePdContaminationinAPIsin30MInutes
- SAMPLE-APIscavengingstepSamples
- SAMPLES/KIT-41inDuplicate
- ADAPTABLE-WorksinHCL,NMP,MeCN,DMF,DMSO,EtOHorToluene
- STABILITY-StableRTLiquidReagents
ThePdX™PallADIumAPIScreeningFluorescentDetectionKitisdesignedtoallowrapidscreeningofmethodsforPdremovalfromsolutionsofAPIsinDMF,Acetonitrile,NMP,Ethanol,diluteHClandToluene.StandardsordilutedsamplesarePipettedintoamicrotiterplatealongwithsuppliedDetectionReagentandSodiumBorohydridereducingagent.After30minutestheintensityofthegeneratedfluorescenceisreadat525nminamicrotiterplatereader.FluorescenceisproportionaltoPdlevel.Noaciddigestionrequired.
Inrecentyears,manynewsynthetictransformationshavebeendevelopedthatusepalladium(Pd)compoundsforthecatalysisofcarbon-carbonandcarbon-heteroatomcouplingreactionssuchastheBuchwald-Hartwig,Heck,Sonogashira,Suzuki-Miyaura,andTsuji-Trosttransformations.Thesereactionshavefoundincreasedpopularityforpharmaceuticalprocessesastheyutilizeawide-rangeoffunctionalgroupstobuildcomplicatedmolecules.Howeverpalladium-catalyzedreactionspresentaprobleminthatthepalladiumcanoftenberetainedintheisolatedAPIproduct.CurrentFDAandEAEMPregulationslimitallplatinumgroup(Pt,Pd,Ir,Rh,Ru,Os)metalcontamination(asagroup)tolessthan5-10ppm.ThestandardmethodsofquantifyingpalladiuminAPIsareatomicabsorptionanalysis,x-rayfluorescence,orinductively-coupledplasmamassspectroscopy(ICP-MS).Theseexpensiveinstrumentsdictateahighlytrainedscientisttooperate,sufferfromcross-contaminationoftheprobeandrequirescrupulouscleanupmethodology.
DataReductionTemplatebyMyAssays