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- MostSensitive
- MultiSpecies
- N-CALKit
- USE-MeasureNitritein5Minutes,TotalNOin30Minutes
- SAMPLE-Water,Serum,Plasma,Urine,Saliva,BuffersandTCM
- SAMPLES/KIT-88inDuplicate
- CALIBRATED-N-Calkit,NIST-Calibrated
- STABILITY-Non-Toxic,Stable4˚CLiquidReagents
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TheDetectX®NitricOxide(NO)DetectionKitisdesignedtoquantitativelymeasureNitrateandNitritepresentinavarietyofsamples.NitricOxidecontentisderivedfromthesumofNitrateandNitrite.BothNitrateandNitritestandardsareprovidedtogeneratestandardcurvesfortheassayandallsamplesshouldbereadofftheappropriatestandardcurve.ForNitriteDetection,samplesaremixedwiththeColorReagentsAandBandincubatedatroomtemperaturefor5minutes.Thecoloredproductisreadat550–570nm.
TotalNitricOxidecontentismeasuredafterthesampleisincubatedwithNitrateReductaseandNADH.ThereductaseincombinationwithNADHreducesNitratetoNitrite.Aftera20minuteincubationatroomtemperature,ColorReagentsAandBareaddedandincubatedatroomtemperaturefor5minutes.TheconcentrationofNitrateinthesampleiscalculatedbytakingthemeasuredNitriteconcentrationandsubtractingfromtheTotalNitricOxideconcentrationforthesample.
DataReductionTemplatebyMyAssays