
- MostSensitive
- MultiSpecies
- USE-MeasureLNGinavarietyofmatrices
- SAMPLE-Saliva,Urine,Water,Milk,TCM,andExtractedSerum,PlasmaorFecalMaterial
- SAMPLES/KIT-40or232inDuplicate
- STABILITY-Liquid4˚CStableReagents
TheDetectX®Levonorgestrel(LNG)ImmunoassaykitisdesignedtoquantitativelymeasureLevonorgestrelpresentinserum,plasma,saliva,urine,driedfecalsamples,water,andtissueculturemediasamples.ThiskitmeasurestotalLNGinextractedserum,plasmaandfecalsamples.
Alevonorgestrelstocksolutionisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturerabbitantibodies.Alevonorgestrel-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofarabbitpolyclonalantibodytolevonorgestreltoeachwell.Afteranhourincubationtheplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundlevonorgestrel-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.Theconcentrationofthelevonorgestrelinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
DataReductionTemplatebyMyAssays