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ARBOR ASSAYS/Glutathione Reductase (GR) Fluorescent Activity Kit/K009-F1/F1

- MostSensitive
- MultiSpecies
- USE-MeasureGRin20MInutesorKinetically
- SAMPLE-Lysate,RBCs,SerumandPlasma
- SAMPLES/KIT-41inDuplicate
- SENSITIVE-9µU/mL
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®GlutathioneReductase(GR)FluorescentActivityKitdeterminesGRactivitybytheamountofGSHgeneratedfromthereductionofGSSGwithanon-fluorescentmolecule,ThioStar®,thatcovalentlybindsthefreethiolgrouponGSHtoyieldahighlyfluorescentproduct.ThemostwidelyusedproceduretomeasureGRistomonitortheoxidationofNADPHasadecreaseinabsorbanceat340nm.HowevermanyBIOLOGicalmoleculesabsorblightat340nm,plusthedetectionsystemgivesverylowODreADIngs.TheARBORASSAYS’DetectX®GlutathioneReductaseFluorescentActivitykitovercomestheseproblemsofmeasuringGRactivitybythedirectdetectionoftheGSHformedfromoxidizedglutathione.
Glutathionereductase(GR)playsanindirectbutessentialroleinthepreventionofoxidativedamagewithinthecellbyhelpingtomaintainappropriatelevelsofintracellularglutathione(GSH).GSH,inconjuctionwiththeenzymeglutathioneperoxidase(GP),istheactingreductantresponsIBLeforminimizingharmfulhydrogenperoxidecellularlevels.TheregenerationofGSHiscatalyzedbyGR.GRisanubiquitous100-120kDadimericflavoproteinthatcatalyzesthereductionofoxidizedglutathione(GSSG)toreducedglutathione,usingß-nicotinamidedinucleotidephosphate(NADPH)asthehydrogendonor.MoleculessuchasNADPHactashydridedonorsinavarietyofenzymaticprocesses.NADPHhasbeensuggestedtoalsoactasanindirectlyoperatingantioxidant,givenitsroleinthere-reductionofGSSGtoGSHandthusmaintainingtheantioxidativepowerofglutathione.
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