
- MostSensitive
- MultiSpecies
- USE-MeasureFreeandTotalGlutathioneinsamewell.
- SAMPLE-Lysate,WB,RBCs,Serum,Plasma,UrineandTissue
- SAMPLES/KIT-39or231inDuplicate
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®Glutathione(GSH)FluorescentDetectionkitutilizesaproprietarynon-fluorescentmolecule,ThioStar®,thatcovalentlybindstothefreethiolgrouponGSHtoyieldahighlyfluorescentproduct.AftermixingthesampleorstandardwithThioStar®andincubatingatroomtemperaturefor15minutes,theGSH-generatedsignalisreadat510nminafluorescentplatereaderwithexcitationat390nm.AdditionofareactionmixtureconvertsalltheGSSGintofreeGSH,whichthenreactswiththeexcessThioStar®duringasecond15minuteincubation,yieldingthesignalrelatedtoTotalGSHcontent.
Glutathione(GSH)isthehighestconcentrationnon-proteinthiolinmammaliancellsandispresentinconcentrationsof0.5to10mM.GSHplaysakeyroleinmanyBIOLOGicalprocesses,includingthesynthesisofproteinsandDNA,thetransportofaminoacids,andtheprotectionofcellsagainstoxidation.Harmfulhydrogenperoxidecellularlevelsareminimizedbytheenzymeglutathioneperoxidase(GP)usingGSHasareductant.TheoxidizedGSHdimer,GSSG,isformedfromGSHandperoxidebytheGPreaction.AnimportantroleofGSSGintheNFkBactivatingsignalcascadeissuggestedbythefactthatthepotentNFkBinducerTPAincreasesintracellularGSSGlevelsandGSSG/GSHratios.
DataReductionTemplatebyMyAssays