
- MultiSpecies
- USE-MeasureGlutathionein20MInutesorKinetically
- SAMPLE-Lysates,WB,RBCs,Serum,Plasma,UrineandTissue
- SAMPLES/KIT-89(OxidizedandTotal)inDuplicate
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®Glutathione(GSH)ColorimetricDetectionkitutilizesacolorimetricsubstratethatreactswiththefreethiolgrouponGSHtoyieldahighlycoloredproduct.Allreagentsareinsolutionandrequiresimpledilutionforuseintheassay.FreeGSHconcentrationinthesampleiscalculatedfromthedifferencebetweenthetotalGSHdeterminedandtheGSHgeneratedfromGSSG.TheconcentrationofGSHcanbedeterminedeitherasanendpointreadofthecolordevelopedat405nmafter30minutesorbymeasuringtherateofcolordevelopmentat405nm.
Glutathione(GSH)isthehighestconcentrationnon-proteinthiolinmammaliancellsandispresentinconcentrationsof0.5to10mM.GSHplaysakeyroleinmanyBIOLOGicalprocesses,includingthesynthesisofproteinsandDNA,thetransportofaminoacids,andtheprotectionofcellsagainstoxidation.Harmfulhydrogenperoxidecellularlevelsareminimizedbytheenzymeglutathioneperoxidase(GP)usingGSHasareductant.TheoxidizedGSHdimer,GSSG,isformedfromGSHandperoxidebytheGPreaction.AnimportantroleofGSSGintheNFkBactivatingsignalcascadeissuggestedbythefactthatthepotentNFkBinducerTPAincreasesintracellularGSSGlevelsandGSSG/GSHratios.
DataReductionTemplatebyMyAssays