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- MostSensitive
- MultiSpecies
- USE-MeasureGlucoseconcentrationin30minutes
- SAMPLE-Serum,Plasma,Urine,BufferandTCM
- SAMPLES/KIT-88induplicate
- SAMPLESIZE-MeasureGlucoseon<1µl>1µl>
- STABILITY-Allliquidreagentsstableat4°C
TheDetectX® GlucoseFluorescentDetectionkitisdesignedtoquantitativelymeasureglucoseinavarietyofsamples. Onlymicroliteramountsofserumorplasmaareneeded.Aß-D-glucosestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. SamplesaremixedwiththeSubstrateandhorserADIshperoxidaseandthereactioninitiatedbyadditionofglucoseoxidase. Thereactionisincubatedatroomtemperaturefor30minutes. Theglucoseoxidasereactswithglucosetoproducehydrogenperoxidewhich,inthepresenceofHRP,reactswiththeSubstratetoconvertthecolorlesssubstrateintoabrightfluorescentproduct. Thefluorescentproductisreadat590nmwithexcitationat570nm.Increasinglevelsofglucosecausealinearincreaseinfluorescence.
ForallBIOLOGicalandmoleculareventsandformultiplecellularfunctions,energyisessential. EnergyisavailableintheformofATP(adenosinetriphosphate),mostofwhichisgeneratedthroughaerobiccellularrespirationofcarbohydrateandglucose,themajorsourceofbiologicalfreeenergyinhigherorganisms.Reducedenergylevelsthreatencellularhomeostasisandintegrity. Impairedenergymetabolismmaytriggerpro-apoptoticsignaling(programmedcelldeath),oxidativedamage,excitotoxicityandimpedemitochondrialDNArepair.
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