
- MostSensitive
- MultiSpecies
- USE-MeasureGlucoseconcentrationin30minutes
- SAMPLE-Serum,Plasma,Urine,BufferandTCM
- SAMPLES/KIT-88induplicate
- SAMPLESIZE-MeasureGlucoseon1µLSerum/Plasma
- STABILITY-Allliquidreagentsstableat4°C
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TheDetectX®GlucoseColorimetricDetectionkitisdesignedtoquantitativelymeasureglucoseinavarietyofsamples. Onlymicroliteramountsofserumorplasmaareneeded.Aß-D-glucosestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. SamplesaremixedwiththeColorimetricSubstrateandhorserADIshperoxidaseandthereactioninitiatedbyadditionofglucoseoxidase. Thereactionisincubatedatroomtemperaturefor30minutes. Theglucoseoxidasereactswithglucosetoproducehydrogenperoxidewhich,inthepresenceofHRP,reactswiththeColorimetricSubstratetoconvertthecolorlesssubstrateintoapink-coloredproduct. Thepinkproductisreadat560nm. Increasinglevelsofglucosecausealinearincreaseincolor.
ForallBIOLOGicalandmoleculareventsandformultiplecellularfunctions,energyisessential. EnergyisavailableintheformofATP(adenosinetriphosphate),mostofwhichisgeneratedthroughaerobiccellularrespirationofcarbohydrateandglucose,themajorsourceofbiologicalfreeenergyinhigherorganisms.Reducedenergylevelsthreatencellularhomeostasisandintegrity. Impairedenergymetabolismmaytriggerpro-apoptoticsignaling(programmedcelldeath),oxidativedamage,excitotoxicityandimpedemitochondrialDNArepair.
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