
- MostSensitive
- MultiSpecies
- USE-MeasureET-1inPlasma,SerumandBIOLOGicalSamples
- SAMPLE-Serum,Plasma,orTCM
- SAMPLES/KIT-39inDuplicate
- SENSITIVE-Measures<0.58=""pg/ml="">
- STABILITY-AllReagentsStableat4˚C
- ECONOMICAL-NoC18Cartridges-SuppliedExtractionSolution
TheDetectX®Endothelin-1(ET-1)ImmunoassaykitisdesignedtoquantitativelymeasureET-1presentinavarietysamplesandtissueculturemedia.AnET-1standardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithamonoclonalantibodytocaptureET-1presentinthesample.Aftera60minuteincubation,theplateiswashedandaperoxidaseconjugatedET-1antibodyisadded.Theplateisagainincubatedfor60minutesandwashed.Substrateisthenaddedtotheplate,whichreactswiththeboundET-1conjugatedantibody.Afterathirdincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheET-1inthesampleisthencalculated.
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