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- MostSensitive
- USE-KidneyInjuryMarker,Resultsin2Hours
- SAMPLE-Serum,Plasma,UrineorTCM
- SAMPLES/KIT-40inDuplicate
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®CystatinCImmunoassayKitisdesignedtomeasureHumanCystatinClevelsinavarietyofBIOLOGicalmatrices.ThiskitusesamicrotiterplatecoatedwithamonoclonaltoCystatinCandanativehumanCystatinCmoleculestandard.SamplesandstandardsareaddedtotheplateandafteranhourincubationatRTtheplateiswashedandmonoclonalantibody-peroxidaseconjugateisadded.Aftera30-minuteincubationatRT,theplateiswashedandTMBsubstratesolutionaddedtoeachwell.Thecolorreactionisreadat450nm.
CystatinCisanon-glycosylatedproteinoflowmolecularweight(13kDa)inthecystatinsuperfamily.CystatinCisproducedataconstantrateinallnucleatedcells.CystatinCbelongstothecysteineproteinaseinhibitorgroupandisassociatedwithseveralpathologicalconditions.ImbalancebetweenCystatinCandcysteineproteinasesisassociatedwithdiseasessuchasinflammation,renalfailure,cancer,Alzheimer’sdisease,multiplesclerosisandhereditaryCystatinCamyloidangiopathy.CystatinCisremovedfrombloodplasmabyglomerularfiltrationinthekidneys.Itisreabsorbedbytheproximaltubularcellsanddegraded.ThereisalinearrelationshipbetweenthereciprocalCystatinCconcentrationinplasmaandtheglomerularfiltrationrate(GFR).CystatinCissuggestedtobeabettermarkerforGFRthanothermarkersasitsserumconcentrationisnotaffectedbyotherfactorssuchasage,genderandbodymass.ThereisassociationofCystatinClevelswiththeincidenceofmyocardialinfarctionandcoronarydeath,presentingariskfactorforsecondarycardiovascularevents.
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