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ARBOR ASSAYS/Cyclic GMP Direct Chemiluminescent Immunoassay Kit/K020-C5/C5

- MostSensitive
- MultiSpecies
- USE-Lyse,StABIlize,MeasurecGMPinonestep
- SAMPLE-Lysate,Urine,Plasma,SalivaandTissue
- SAMPLES/KIT-39or231inDuplicate
- SENSITIVITY-MeasuresasLittleas1fmolcGMPpersample
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®DirectCyclicGMP(cGMP)ChemiluminescentImmunoassay(CLIA)kitwillmeasurecGMPpresentinlysedcells,EDTAplasma,urine,salivaandculturemediasamples.ThekitisuniqueinthatallsamplesandstandardsaredilutedintoanacidicSampleDiluent,whichcontainsspecialadditivesandstabilizers,forcGMPmeasurement.AcidifiedsamplesofcGMParestableandendogenousphosphodiesterasesareinactivatedintheSampleDiluent.Afteranovernightincubationat4°C,theplateiswashedandthechemiluminescentsubstrateisadded.Thesubstrategenerateslightwhichisdetectedinamulti-labelmicrotiterplatereadercapableofreADIngluminescence.
CyclicGMPisacriticalandmultifunctionalsecondmessengerpresentatlevelstypically10-100foldlowerthancAMPinmosttissues.IntracellularcGMPisformedbytheactionoftheenzymeguanylatecyclaseonGTPanddegradedthroughphosphodiesterasehydrolysis.Guanylatecyclases(GC)areeithersolubleormembranebound.SolubleGCsarenitricoxideresponsive,whereasmembraneboundGCsrespondtohormonessuchasacetylcholine,insulinandoxytocin.OtherchemicalslikeSEROtoninandhistaminealsocauseanincreaseincGMPlevels.CyclicGMPregulatescellularcompositionthroughcGMP-dependentkinase,cGMP-dependentionchannelsortransporters,andthroughitshydrolyticdegradationbyphosphodiesterase.
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