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ARBOR ASSAYS/Cyclic AMP Direct Chemiluminescent Immunoassay Kit/K019-C5/C5

- MostSensitive
- MultiSpecies
- USE-MeasurecAMPin2hours
- SAMPLE-Lysates,Urine,Plasma,Saliva,Tissue
- SAMPLES/KIT-38or230inDuplicate
- SENSITIVITY-Measure<1=""fmol=""camp=""per="">
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®CyclicAMP(cAMP)ChemiluminescentDirectImmunoassay(CLIA)kitsaredesignedtoquantitativelymeasurecAMPpresentincelllysates,plasma,urine,saliva,tissueandculturemediasamples.ThesuppliedSampleDiluentwilllysecells,stabilizecAMPandstopphosphodiesteraseactivity.AcAMPstandardisprovidedtogenerateastandardcurvefortheassay.ThesuppliedPlatePrimersolutionisaddedtothewellsofacoatedwhitemicrotiterplate,followedbystandardsordilutedsamples.AcAMP-peroxidaseconjugateisthenaddedtothewells.
ThebindingreactionisinitiatedbytheadditionofapolyclonalantibodytocAMP.Aftera2hourincubationtheplateiswashedandchemiluminescentsubstrateisadded.ThesubstrateimmediatelyreactswiththeboundcAMP-peroxidaseconjugate.Thegeneratedchemiluminescentglowsignalismeasured.TheconcentrationofthecAMPinthesampleiscalculated,aftermakingcorrectionforthedilution.
CyclicAMP(cAMP)isoneofthemostimportantsecondmessengersandakeyintracellularregulator.DiscoveredbySutherlandandRallin1957,itfunctionsasamediatorofactivityforanumberofhormones,includingepinephrine,glucagon,andACTH.CyclicAMPisproducedbytheenzymeadenylatecyclase,andtheenzymeisactivatedbythehormonesglucagonandadrenalineandbyGprotein.cAMPdecompositionintoAMPiscatalyzedbytheenzymephosphodiesterase.OtherBIOLOGicalactionsofcAMPincluderegulationofinnateimmunefunctioning,axonregeneration,cancer,andinflammation.
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