
- MultiSpecies
- N-CALKit
- USE-UrineVolume
- SAMPLE-Urine
- SAMPLES/KIT-88or472inDuplicate
- CALIBRATED-N-CalKit,NIST-Calibrated
- STABILITY-Liquid4°CStableReagents
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TheDetectX®UrinaryCreatinineDetectionkitsaredesignedtoquantitativelymeasurecreatinineinurinesamples.ANISTcalibratedcreatininestandardisusedtostandardizetheassay.SamplesarePipettedintoaclearmicrotiterplateandthecolorgeneratingreactionisinitiatedwiththesuppliedno-mixDetectXCreatinineReagent,whichispipettedintoeachwell.Aftera30minuteincubationatroomtemperaturethecolorisreadat490nm.
Creatinine(2-amino-1-methyl-5H-imadazol-4-one)isametaboliteofphosphocreatine(p-creatine),amoleculeusedasastoreforhigh-energyphosphatethatcanbeutilizedbytissuesfortheproductionofATP.Creatineeithercomesfromthedietorsynthesizedfromtheaminoacidsarginine,glycine,andmethionine. Creatineandp-creatineareconvertednon-enzymaticallytothemetabolitecreatinine,whichdiffusesintothebloodandisexcretedbythekidneys.Invivo,thisconversionappearstobeirreversIBLeandinvitroitisfavoredbyhighertemperaturesandlowerpH.Creatinineformsspontaneouslyfromp-creatine.Undernormalconditions,itsformationoccursataratethatisrelativelyconstantandasintra-individualvariationis <15%fromdaytoday,creatinineisausefultoolfornormalizingthelevelsofothermoleculesfoundinurine.
DataReductionTemplatebyMyAssays