
- MultiSpecies
- N-CALKit
- USE-KidneyDamage
- SAMPLE-SerumandPlasma
- SAMPLES/KIT-91or187inDuplicate
- CALIBRATED-N-CalKit,NIST-Calibrated
- STABILITY-Liquid4°CStableReagents
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TheDetectX®SerumCreatinineKitmeasurescreatinineinserumandplasmasamples.Acreatininestandard,calibratedtoaNISTcreatininestandard,isprovidedtogenerateastandardcurve.ThecolorgeneratingreactionisinitiatedwiththeCreatinineDetectionReagent.Theassayutilizesakineticabsorbancemethodtoovercomeinterferencebycoloredcompoundsinthesample.TheconcentrationofcreatinineiscalculatedusingthedeltaoftheopticaldensityreADIngsat1and30minutes.WeofferafreeExcelworksheetforconcentrationcalculations.
Creatinineisametaboliteofphosphocreatine(p-creatine),ahigh-energyphosphatethatcanbeutilizedbytissuesfortheproductionofATP.Creatineeithercomesfromthedietorsynthesizedfromtheaminoacidsarginine,glycine,andmethionine.Creatineandp-creatineareconvertednon-enzymaticallytothemetabolitecreatinine,whichdiffusesintothebloodandisexcretedbythekidneys.Invivo,thisconversionappearstobeirreversIBLeandinvitroitisfavoredbyhighertemperaturesandlowerpH.Measuringserumcreatinineisoneofthemostcommonlyusedindicatorsofrenalfunction.Ariseinbloodcreatininelevelsisobservedonlywithmarkeddamagetofunctioningnephrons.Agoodindicatorofkidneyfunctionisgivenbythecreatinineclearancetest.Creatinineclearancecanbeaccuratelycalculatedusingserumcreatinineconcentrationandtakingintoaccountthevariablesofsex,age,weight,andrace.
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