
- MultiSpecies
- USE-MeasuretheActivityof11B-HSDEnzymesin2hours
- SAMPLE-Plasma,Serum,Saliva,Urine,Media,andFecalExtracts
- SAMPLES/KIT-37or229inDuplicate
- STABILITY-Stable4˚CLiquidReagents
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TheDetectX®CortisoneChemiluminescentImmunoassay(CLIA)kitisdesignedtoquantitativelymeasureCortisonepresentinsamples.Acortisonestandardisprovidedtogenerateastandardcurvefortheassay.Afteratwohourincubationtheplateiswashedandthechemiluminescentsubstrateisadded.Thesubstratereactswiththeboundcortisone-peroxidaseconjugatetoproducelight.Thegeneratedlightisdetectedinamultilabelmicrotiterplatereader.
Cortisoneandcortisolconcentrationsvaryduetotheactivityoftwo11ß-hydroxysteroiddehydrogenases(11-HSD).Whilemosttissueshavetheabilitytoexpresseitherenzyme,11ß-HSD1isfoundprimarilyintheliverwhereitconvertscortisonetocortisolwhile11ß-HSD2isfoundintissuessuchasthekidneywherecortisolreceptorbindingisrequired.11ß-HSD2deactivatescortisoltocortisone,prohibitingreceptoractivation.Thisglucocorticoid“shuttle”helpstoinitiateandregulatetheanti-inflammatoryresponse.Monitoringtheratioofcortisone:cortisolhasapplicationsindiabetes,obesity,metabolicsyndrome,osteoporosis,andchronicfatiguesyndromeinadditiontoadrenaldiseases.Cortisoneandcortisolconcentrationsexhibitapredictablediurnalpatternandcanbemeasuredinextracteddriedfeces,orinserum,plasma,salivaandurine.ArecentpublicationhassuggestedthatsalivarycortisoneisagoodsurrogateMarkerforserumcortisol.
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