
- MostSensitive
- MultiSpecies
- USE-EverythingneededtomeasureCatalaseactivityin45Minutes
- SAMPLE-MeasureCatalaseActivityinanySample
- SAMPLES/KIT-89inDuplicates
- STABILITY-Liquid4°Cstablereagents
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TheDetectX®CatalaseActivityKitisdesignedtoquantitativelymeasurecatalaseactivityinavarietyofsamples.Abovinecatalasestandardisprovidedtogenerateastandardcurvefortheassay.SamplesaredilutedintheprovidedAssayBufferandaddedtothewellsofahalfareablackplate.Hydrogenperoxideisaddedtoeachwellandtheplateincubatedatroomtemperaturefor30minutes.ThesuppliedFluorescentdetectionReagentisadded,followedbydilutedhorserADIshperoxidaseandincubatedatroomtemperaturefor15minutes.TheHRPreactswiththesubstrateinthepresenceofhydrogenperoxidetoconvertthecolorlesssubstrateintoafluorescentproduct.Thefluorescentproductisreadat590nmwithexcitationat570nm.IncreasinglevelsofcatalaseinthesamplescausesadecreaseinH2O2concentrationandareductioninfluorescentproduct.TheactivityofthecatalaseinthesampleareexpressedintermsofunitsofcatalaseactivitypermL.
DataReductionTemplatebyMyAssays