
- MostSensitive
- MultiSpecies
- MULTI-SPECIES-MeasuresAVPinmammals,Arg-Vasotocininbirdsandreptiles
- SENSITIVITY-Measure<0.9 pg/ml="" most="">0.9>
- SIMPLE-NoC18ColumnPurifications
- INEXPENSIVE-ExtractionSolutionIncluded
- SAMPLES/KIT-38or230inDuplicate
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TheArg8-Vasopressin(AVP)ChemiluminescentImmunoassay(CLIA)kitisdesignedtoquantitativelymeasureAVPpresentinserum,plasmaandtissueculturemediasamples.AnAVPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. StandardsordilutedsamplesarePipettedintoawhitemicrotiterplatecoatedwithanantibodytocapturerabbitantibodies. AnAVP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells. ThebindingreactionisinitiatedbytheadditionofapolyclonalantibodytoAVPtoeachwell. Afteranovernightincubationat4°Ctheplateiswashedandsuppliedsubstrateisadded. ThesubstratereactswiththeboundAVP-peroxidaseconjugate. Theintensityofthegeneratedchemiluminescentsignalisdetectedinamicrotiterplatereadercapableofmeasuringluminescence. TheconcentrationoftheAVPinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
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