The Arg8-Vasopressin (AVP) Chemiluminescent Immunoassay (CLIA) Kit is designed to quantitatively measure AVP present in serum, plasma and tissue culture media samples. An AVP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit antibodies. An AVP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to AVP to each well. After an overnight incubation at 4°C the plate is washed and supplied substrate is added. The substrate reacts with the bound AVP-peroxidase conjugate. The intensity of the generated chemiluminescent signal is detected in a microtiter plate reader capable of measuring luminescence. The concentration of the AVP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
AVP Standard Curve
