- MostSensitive
 - MultiSpecies
 
- USE-Measureallopregnanoloneinavarietyofsamples
 - SAMPLE-Urine,TCMorExtractedSerum,Plasma,andDriedFeces
 - SAMPLES/KIT-39or231inDuplicate
 - SENSITIVITY-129.7pg/mL
 - SELECTIVE-LowCrossReactivitytoProgesteroneandMetabolites
 
ViewPublications
AllopregnanoloneaidsneurogenesisandhasbeenfoundtoreverseneuronproliferativedeficitandcognitivedeficitsinamousemodelofAlzheimer’sdisease. AllopregnanolonehasalsobeenshowntorestorefunctionalityinamousemodelofParkinson’sdiseaseandtoimprovebehavioralproblemsinpost-traumaticstressdisorder.
TheDetectX®AllopregnanoloneImmunoassaykitisdesignedtoquantitativelymeasureallopregnanolonepresentinextractedserum,plasma,driedfecalsamples,ordilutedurineandtissueculturemediasamples. Pleasereadthecompletekitinsertbeforeperformingthisassay. Anallopregnanolonestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. StandardsordilutedsamplesarePipettedintoaclearmicrometerplatecoatedwithanantibodytocapturemouseantibodies. Anallopregnanolone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells. Thebindingreactionisinitiatedbytheadditionofamonoclonalantibodytoallopregnanolonetoeachwell.
Thekithastwoformatoptions:aprimaryincubationoftwohoursatroomtemperaturewithshaking,orovernightat4°C. Attheendoftheincubationperiodtheplateiswashedandsubstrateisadded. Thesubstratereactswiththeboundallopregnanolone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength. Theconcentrationoftheallopregnanoloneinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
 

		