
- MostSensitive
- MultiSpecies
- USE-Measureallopregnanoloneinavarietyofsamples
- SAMPLE-Urine,TCMorExtractedSerum,Plasma,andDriedFeces
- SAMPLES/KIT-39or231inDuplicate
- SENSITIVITY-129.7pg/mL
- SELECTIVE-LowCrossReactivitytoProgesteroneandMetabolites
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AllopregnanoloneaidsneurogenesisandhasbeenfoundtoreverseneuronproliferativedeficitandcognitivedeficitsinamousemodelofAlzheimer’sdisease. AllopregnanolonehasalsobeenshowntorestorefunctionalityinamousemodelofParkinson’sdiseaseandtoimprovebehavioralproblemsinpost-traumaticstressdisorder.
TheDetectX®AllopregnanoloneImmunoassaykitisdesignedtoquantitativelymeasureallopregnanolonepresentinextractedserum,plasma,driedfecalsamples,ordilutedurineandtissueculturemediasamples. Pleasereadthecompletekitinsertbeforeperformingthisassay. Anallopregnanolonestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. StandardsordilutedsamplesarePipettedintoaclearmicrometerplatecoatedwithanantibodytocapturemouseantibodies. Anallopregnanolone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells. Thebindingreactionisinitiatedbytheadditionofamonoclonalantibodytoallopregnanolonetoeachwell.
Thekithastwoformatoptions:aprimaryincubationoftwohoursatroomtemperaturewithshaking,orovernightat4°C. Attheendoftheincubationperiodtheplateiswashedandsubstrateisadded. Thesubstratereactswiththeboundallopregnanolone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength. Theconcentrationoftheallopregnanoloneinthesampleiscalculated,aftermakingsuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.