
- MultiSpecies
- USE-MeasureAlloprenanolonein2.5Hours
- SAMPLE-ExtractedSerum,Plasma,Fecal,andUrineorTCM
- SAMPLES/KIT-38or230inDuplicate
- SELECTIVE-LowCrossReactivitytoProgesteroneandMetabolites
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TheDetectX®AllopregnanoloneImmunoassaykitisdesignedtoquantitativelymeasureAllopregnanolonepresentinextractedserum,plasma,anddriedfecalsamples,andinurineandtissueculturemediasamples.Anallopregnanolonestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturerabbitantibodies.Anallopregnanolone-peroxidaseconjugateisaddedtothewells.ThebindingreactionfortheEIA/ELISAisinitiatedbytheadditionofapolyclonalantibodytoallopregnanolonetoeachwell.Afterincubationtheplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundallopregnanolone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorfromtheEIA/ELISAisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.
AllopregnanoloneaidsneurogenesisandhasbeenfoundtoreverseneuronproliferativedeficitandcognitivedeficitsinamousemodelofAlzheimer’sdisease.AllopregnanolonehasalsobeenshowntorestorefunctionalityinamousemodelofParkinson’sDisease.Ithasalsobeenshowntoimprovebehavioralproblemsinpost-traumaticstressdisorder.
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