- MultiSpecies
- SENSITIVE-Measurefrom5,000to78.125pg/mL
- SAMPLE-ExtractedSerum,Plasma,Fecal,andUrineorTCM
- SAMPLES/KIT-39or231inDuplicate
- SELECTIVE-LowCrossReactivitytoProgesteroneandMetabolites
TheDetectX®AllopregnanoloneChemiluminescentImmunoassay(CLIA)kitisdesignedtoquantitativelymeasureAllopregnanolonepresentinextractedserum,plasma,ordriedfecalsamples,orindilutedurineandtissueculturemediasamples. Anallopregnanolonestandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve. StandardsorsamplesarePipettedintoawhitemicrotiterplatecoatedwithantibodytocapturerabbitantibodies. Anallopregnanolone-peroxidaseconjugateisaddedtothewellsandthebindingreactionisinitiatedbyadditionofapolyclonalantibodytoallopregnanolonetoeachwell. Theassayisincubatedovernightat4˚C. Attheendoftheincubationsperiodtheplateiswashedandchemiluminescentsubstrateisadded. Thechemiluminescentsubstratereactswiththeboundallopregnanolone-peroxidaseconjugatetogeneratelight.
AllopregnanoloneaidsneurogenesisandhasbeenfoundtoreverseneuronproliferativedeficitandcognitivedeficitsinamousemodelofAlzheimer’sdisease.AllopregnanolonehasalsobeenshowntorestorefunctionalityinamousemodelofParkinson’sDisease.Ithasalsobeenshowntoimprovebehavioralproblemsinpost-traumaticstressdisorder.
DataReductionTemplatebyMyAssays