
- MostSensitive
- MultiSpecies
- SENSITIVE-Measurefrom5,000to3.9pg/mL
- SAMPLE-Serum,EDTAorHeparinPlasma,Urine,FecalExtractsandTCM
- SAMPLES/KIT-40or232inDuplicate
- STABILITY-Liquid4˚CStableReagents
- DUALRANGE-4,000to3.9or5,000to8.2pg/mL
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TheDetectX®AldosteroneEnzymeImmunoassaykitisdesignedtoquantitativelymeasureAldosteronepresentinextractedserumandplasma,orinurine,extracteddriedfecalsamples,andtissueculturemediasamples. Analdosteronestocksolutionisprovidedtogenerateastandardcurve.Protocolsareprovidedtoprepareassaystandardsfrom4,000to3.906pg/mLorfrom5,000to8.192pg/mL. SamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturesheepantibodies.Analdosterone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofapolyclonalantibodytoaldosteronetoeachwell.Afterincubation,theplateiswashedandsubstrateisadded.Thesubstratereactswiththeboundaldosterone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedat450nm.
DataReductionTemplatebyMyAssays