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- MostSensitive
- MultiSpecies
- SENSITIVE-Measurefrom1,500to2.06pg/mL
- SAMPLE-Serum,EDTAorHeparinPlasma,Urine,FecalExtractsandTCM
- SAMPLES/KIT-39or231inDuplicate
- STABILITY-Liquid4˚CStableReagents
TheDetectX®AldosteroneChemluminescentImmunoassay(CLIA)kitisdesignedtoquantitativelymeasureAldosteronepresentinextractedserumandplasma,orinurine,extracteddriedfecalsamples,andtissueculturemediasamples. Analdosteronestocksolutionisprovidedtogenerateastandardcurve.Thekithasastandardrangefrom1,500to2.06pg/mL. SamplesarePipettedintoawhitemicrotiterplatecoatedwithanantibodytocapturesheepantibodies.Analdosterone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofapolyclonalantibodytoaldosteronetoeachwell.Afterincubationovernightat4°C,theplateiswashedand chemiluminescentsubstrateisadded.Thesubstratereactswiththeboundaldosterone-peroxidaseconjugate.Thesubstratereactswiththeboundcorticosterone-peroxidaseconjugatetoproducelight.
DataReductionTemplatebyMyAssays