The DetectX® Aldosterone Chemluminescent Immunoassay (CLIA) kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. An aldosterone stock solution is provided to generate a standard curve. The kit has a standard range from 1,500 to 2.06 pg/mL. Samples are pipetted into a white microtiter plate coated with an antibody to capture sheep antibodies. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After incubation overnight at 4°C, the plate is washed and chemiluminescent substrate is added. The substrate reacts with the bound aldosterone-peroxidase conjugate. The substrate reacts with the bound corticosterone-peroxidase conjugate to produce light.
