
- MostSensitive
- MultiSpecies
- SENSITIVE-Measurefrom6,000to24.7pg/mL
- SAMPLE-ExtractedSerumandPlasma,Urine,FecalExtractsandTCM
- SAMPLES/KIT-40or232inDuplicate
- STABILITY-Liquid4˚CStableReagents
TheDetectX®17-HydroxyprogesteroneEnzymeImmunoassaykitisdesignedtoquantitativelymeasure17-Hydroxyprogesteronepresentinextractedserumandplasma,orinurine,extracteddriedfecalsamples,andtissueculturemediasamples. A17-Hydroxyprogesteronestocksolutionisprovidedtogenerateastandardcurve.Protocolsareprovidedtoprepareassaystandardsfrom6,000to24.7pg/mL. SamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturesheepantibodies.A17-hydroxyprogesterone-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.Thebindingreactionisinitiatedbytheadditionofapolyclonalantibodyto17-hydroxyprogesteronetoeachwell.Afteranhourincubation,theplateiswashedandsubstrateisadded.Thesubstratereactswiththebound17-hydroxyprogesterone-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedat450nm.
DataReductionTemplatebyMyAssays