The DetectX® 17-Hydroxyprogesterone Enzyme Immunoassay kit is designed to quantitatively measure 17-Hydroxyprogesterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. A 17-Hydroxyprogesterone stock solution is provided to generate a standard curve. Protocols are provided to prepare assay standards from 6,000 to 24.7 pg/mL. Samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A 17-hydroxyprogesterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17-hydroxyprogesterone to each well. After an hour incubation, the plate is washed and substrate is added. The substrate reacts with the bound 17-hydroxyprogesterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected at 450nm.
