
- Purifiedtohomogeneityfromthejellyfish,Aequoreavictoria
- RecombinantwildtypeproteinexpressedinE.coli
- Finalhighresolutiongelfiltrationpurified
Greenfluorescentprotein(GFP),a27kDaproteinderivedfromthejellyfishAequoreavictoria,emitsgreenlight(emissionpeak509nm)whenexcitedbybluelight(excitationpeak395nm).GFPhasbecomeaninvaluabletoolincellBIOLOGyresearch,sinceitsintrinsicfluorescencecanbevisualizedinlivingcells.GFPfluorescenceisstableunderfixationconditionsandsuitableforavarietyofapplications.GFPhasbeenwidelyusedasareporterforgeneexpression,enablingresearcherstovisualizeandlocalizeGFP-taggedproteinswithinlivingcellswithouttheneedforchemicalstaining.OtherapplicationsofGFPincludeassessmentofprotein-proteininteractionsthroughtheyeasttwohybridsystemandmeasurementofdistancebetweenproteinsthroughfluorescenceenergytransfer(FRET)protocols.GFPisusedtomeasuresinglecellmetastasisandsuccessfulproliferationofstemcells.